Multiplex PCR-based strategy to detect contamination with mycotoxigenic Fusarium species in rice and fingermillet collected from southern India

BACKGROUND: The genus Fusarium comprises a diverse group of fungi including several species that produce mycotoxins in food commodities. In the present study, a multiplex PCR was standardised for the group‐specific detection of fumonisin‐producing and trichothecene‐producing strains of Fusarium species. Primers for genus‐level recognition of Fusarium spp. were designed from the internal transcribed spacer regions 1 and 2 of rDNA. Primers for group‐specific detection were designed from the tri5 and tri6 genes involved in trichothecene biosynthesis and the fum1 and fum13 genes involved in fumonisin biosynthesis. RESULTS: Among the various genera and their strains tested, all the 85 confirmed Fusarium strains were positive for rDNA gene and the rest stayed negative. From among the Fusarium strains, 15 had amplification for trichothecene‐ and 20 for fumonisin‐encoding genes. All PCR positive trichothecene chemotypes of Fusarium species tested were positive for chemical analysis but in the case of fumonisins, of the 20 PCR positive cultures, only 13 showed positive for chemical analysis by HPTLC. CONCLUSION: The assay described here provided a rapid and reliable detection of trichothecene‐ and fumonisin‐producing Fusarium directly from natural food grains and the results were always comparable with a conventional HPTLC detection method. It can, therefore, be used by the food industry to monitor quality and safety. Copyright © 2011 Society of Chemical Industry     

Analysis and simulation of a solar water pump for lift irrigation

A solar water pump for lift irrigation, which was shown to be economically viable, was proposed by Rao and Rao [5]. A “modified pump” is suggested, which is suitable for village water supply. The thermodynamic analysis of the pumps is presented. Though the solar water pump is intended to be operated with flat-plate collectors, it is analysed whether the pump could be run more efficiently when coupled with concentrating collectors. The analysis is also applicable for bellow actuated solar water pumps.Preliminary experimental studies showed that the heat losses are 2–3 times the theoretical energy requirement and the losses to the water tank shell accounted for a major part of the total heat losses. To reduce these losses, it is proposed that the inner surface of the water tank shell be lined with a resin bonded cork insulation. A method to evaluate the heat losses to the shell with insulation by solving the unsteady state heat conduction equation for a composite cylindrical body with time varying convective boundary conditions is presented. The heat losses are reduced to 5–15 per cent of the theoretical energy requirement with the use of the internal insulation. The major problem encountered in operating the pump was the inadequate condensation of the working fluid, which resulted in failure of the suction of water into the water tank. To ensure proper suction of water, the conditions to be maintained are analysed. The presence of water vapor and air in the spent vapor has to be taken into consideration while designing the condenser.An algorithm to evaluate the year round performance of the water-cooled pump for any location, given the lift and collector area, is presented. The cost of the water-cooled pump is compared with the costs of other types of solar water pumps and it is shown that the pump under consideration costs several times less than the other ones. The conditions at which the pump will be economical vis-a-vis diesel and electrical pumps are presented.     

A study on the sealing performance of bolted flange joints with gaskets using finite element analysis

Gaskets play an important role in the sealing performance of bolted flange joints, and their behaviour is complex due to nonlinear material properties combined with permanent deformation. The variation of contact stresses due to the rotation of the flange and the material properties of the gasket play important roles in achieving a leak proof joint. In this paper, a three-dimensional finite element analysis (FEA) of bolted flange joints has been carried out by taking experimentally obtained loading and unloading characteristics of the gaskets. Analysis shows that the distribution of contact stress has a more dominant effect on sealing performance than the limit on flange rotation specified by ASME.     

Molecular recognition of the Lewis Y antigen by monoclonal antibodies

The murine monoclonal antibody BR55-2 is directed against thetumor-associated antigen Lewis Y oligosaccharide. The LewisY core antigen is a difucosylated structure consisting of fourhexose units. Analysis of binding profiles of lactoseries isomericstructures by BR55–2 suggest that the binding epitopeincludes the OH-4 and OH-3 groups of the ß-D-galactoseunit, the 6-CH₃ groups of the two fucose units and the N-acetylgroup of the subterminal ß-D-N-acetylglucosamine (ßDGlcNAc).To elucidate the molecular recognition properties of BR55–2for the Y antigen, BR55–2 was cloned, sequenced and itsthree-dimensional structure was examined by molecular modeling.The crystal structure of BR96, another anti-Lewis Y antibody,solved in complex with a nonoate methyl ester Lewis Y tetrasaccharide,and the lectin IV protein in complex with a Lewis b tetrasaccharidecore were used as a guide to probe the molecular basis for BR55–2antigen recognition and specificity. Our modeling study showsthat BR55–2 shares similar recognition features for thedifucosylated type 2 lactoseries Lewis Y structure observedin the BR96-sugar complex. We observe that a major source ofspecificity for the Lewis Y structure by anti-Y antibodies emanatesfrom interaction with the ß-D-N-acetylglucosamineresidue and the nature of the structures extended at the reducingsite of the fucosylated lactosoamine.     

Synthetic CD4 exocyclic peptides antagonize CD4 holoreceptor binding and T cell activation

We have developed peptide analogs to analyze precise human CD4 substructures involved in MHC class II binding. Forms of the complementarity determining-like regions (CDRs) of the D1 domain of human CD4 were reproduced as synthetic aromatically modified exocyclic (AME) analogs and tested for their ability to block CD4-MHC II interactions and T cell activation. The exocyclic derived from CDR3 (residues 82-89) of human CD4, which specifically associated with CD4 on the T cell surface to create a heteromeric CD4 complex, blocked IL-2 production and antagonized the normal function of the CD4 receptor. The approach of creating novel synthetic antagonistic receptor complexes may represent a new receptor specific pharmaceutical approach to modulate biological function.     

Depth from defocus: A spatial domain approach

A new method named STM is described for determining distance of objects and rapid autofocusing of camera systems. STM uses image defocus information and is based on a new Spatial-Domain Convolution/Deconvolution Transform. The method requires only two images taken with different camera parameters such as lens position, focal length, and aperture diameter. Both images can be arbitrarily blurred and neither of them needs to be a focused image. Therefore STM is very fast in comparison with Depth-from-Focus methods which search for the lens position or focal length of best focus. The method involves simple local operations and can be easily implemented in parallel to obtain the depth-map of a scene. STM has been implemented on an actual camera system named SPARCS. Experiments on the performance of STM and their results on real-world planar objects are presented. The results indicate that the accuracy of STM compares well with Depth-from-Focus methods and is useful in practical applications. The utility of the method is demonstrated for rapid autofocusing of electronic cameras.     

Thermal distribution calculations for block level placement in embedded systems

Reliability is a very important concern for the embedded systems. Thermal distribution has become an important reliability concern for today’s integrated circuits and these circuits are being used increasingly in embedded systems. In traditional design flows, the temperature of the chip is assumed to be uniform across the substrate. However, non-uniform thermal distribution can be a major source of inaccuracy in delay and clock skew computations, and can have an impact on elctromigration reliability and self-heating effects for today’s very deep submicron technology. Hence, it has become necessary to obtain design with uniform temperature distribution to ensure minimum temperature gradient and avoid hot spots across the chip area. This will minimise reliability problems during the operation of the chip. The uniform temperature distribution can be achieved by appropriate placement of circuit blocks during the physical design. In this paper, thermal distribution of single chip embedded system on silicon is discussed. The thermal distribution calculations require evaluation of switching activity factor of circuit blocks. This factor is determined by computing activities of the blocks based on the application software of embedded system.     

Molecular recognition of a peptide mimic of the Lewis Y antigen by an anti-Lewis Y antibody

Peptides as mimics of carbohydrates display a distinct advantage in vaccine design because of ease of synthesis and their inherent T cell-dependent nature as immunogens. While peptides that mimic carbohydrates have been described, it is not clear how they do so. To further our insight into structural relationships between peptide-mimics and carbohydrate structures, we have analyzed a potential recognition scheme between the murine monoclonal antibody, B3, directed against the tumor-associated antigen Lewis Y oligosaccharide and a peptide identified from phage display screening with B3. The Lewis Y core antigen is a difucosylated structure consisting of four hexose units. The B3 antibody binds to the peptide sequence APWLYGPA in which the putative sequence APWLY is critical for binding to the antibody. Not having experimental structural information for B3, the crystal structure of another anti-Lewis Y antibody, BR96, solved in complex with a nonoate methyl ester Lewis Y tetrasaccharide, provides a molecular basis for LeY antigen recognition and specificity, and how this binding relates to peptide binding. As a guide to place the APWLY motif in the B3 combining site, a fragment library was searched for analogous compounds that have the potential to bind to B3. Our modeling study shows that the B3-peptide complex shares similar recognition features for the difucosylated type 2 lactoseries' structure. This analysis provides a molecular perspective for peptide mimicry of a carbohydrate epitope.     

Growth of cadmium selenide layers by electrodeposition

Cadmium selenide (CdSe) films have been electrodeposited from a bath containing CdSO₄ and SeO₂. The pH of the bath was around 2. The deposition was done by short circuiting different substrates like Indium Tin Oxide ITO, molybdenum, sodium selenosulphite etc. to an easily oxidizable electrode (such as Al/Cd) in an electrolytic bath. The temperature of the deposition bath was varied in the range 60 to 85 ° C. The as-grown films were characterized by X-ray diffraction and scanning electron microscopy. Electrical characterization of the ITO/ CdSe/In structure was made by studying the current-voltage characteristics. Optical absorption measurements yielded a direct band gap around 1.65eV.